ISOLATION AND CHARACTERIZATION OF NITROGEN AND INDOLE ACETIC ACID PRODUCING RHIZOBACTERIA FROM LOCAL UPLAND RICE PLANTS IN SOUTH SULAWESI, INDONESIA
This study aimed to determine the type of isolate and the characteristics of nitrogen fixing bacteria in the rhizosphere of local organic upland rice in the highlands. This research was conducted in North Luwu Regency, South Sulawesi, Indonesia and the Pest Science Laboratory, Department of Plant Pests and Diseases, Hasanuddin University. The research method was a descriptive study using morphological and physiological characterization (Gram reaction test using 3% KOH), nitrogen fixation ability test of bacteria and Indole Acetic Acid (IAA) production test. Morphological characterization of nitrogen fixing bacterial isolates from local organic rice rhizospheres showed 5 of the tarone variety and 7 of the local rice tarone hoyane variety from Seko Subdistrict. The results of the morphological characterization showed different results in terms of color, size, shape and level. Nitrogen fixation ability test of the isolate from the rhizosphere of the local rice tarone hoyane variety achieved the highest nitrogen binding ability (2.082), Seko Subdistrict (962), Rongkong Subdistrict (996), and the lowest was the isolate from the banjara rice variety (707). The ability test of IAA production of the local upland rice rhizosphere bacterial isolates of the tarone hoyane rice variety produced a more intense pink color and had the highest concentration of IAA (1.835 mg L-1), Seko Subdistrict (1.630 mg L-1), and Rongkong Subdistrict (1,566 mg L-1). Meanwhile, the bacterial isolate of the local rice banjara variety from Rongkong Subdistrict obtained the lowest IAA (0.316 mg L-1).
Indole Acetic Acid; Nitrogen; Rhizobacteria; Rice