COMPARISON BETWEEN SOME METABOLIC HORMONES AND BIOCHEMICAL PARAMETERS BEFORE, DURING AND AFTER PUBERTY IN KARADI EWE LAMBS UNDER NATURAL CONDITIONS IN KURDISTAN REGION OF IRAQ
The aim of this paper is to determine the concentration of blood metabolic hormones (leptin, insulin and estradiol) levels in growing Karadi Ewe lambs. The research was carried out at bamerne Village, Duhok governorate, Kurdistan region of Iraq. Twenty four Ewe lambs randomly selected as per age and body weight were divided into four groups, Group I (>3 to 5 months)( 20±0.4 or 16±1.5 Kg), Group II (>5 to 7 months)( 26.4±1.2 or 26±4.4 Kg), Group III (>7 to 9 months) (34± 2.023 Kg) and Group IV (>9 to 11 months)( 40.562± 2.202 Kg), with six animals in each group .Concentrations of insulin, leptin and estradiol were determined in the blood serum of ewe lambs during (pre pubertal puberty and post pubertal ewes) were determined by ELISA using ovine kits. The concentration of insulin decreased with increasing age, the level of estradiol elevated at the third groups it means the pubertal age in Karadi ewe, non- significant elevation of leptin concentration with increasing age. The serum biochemical parameters measured in the study included lipid profile (cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL), triglycerides), Total protein profile (TP) serum albumin, serum globulin, carbohydrate profile. Total protein increased, but by a small percentage, compared to the first group, the lipid and carbohydrate profile gradually decreased with increasing age. This decreasing in the percentage of insulin due to feeding on herbs and the weather conditions of the region. The aim of the current study is to estimate the baseline information of physiological concentration and relation of metabolic hormones leptin, insulin and estrogen levels before, during and after puberty in Karadi Ewe lambs under natural conditions of Iraqi Kurdistan region.
Leptin; Insulin; Estradiol; Metabolic Hormones; Total Protein; Lipid Profile; Karadi Ewe; Puberty.