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Original Research

UTILIZATION OF ALGAE SPIRULINA sp. AS A TOXICITY REDUCETING AGENT FOR HEAVY METALS Fe, Zn AND DYE PROCION RED, METHYL ORANGE, DIRECT GREEN AND NAPTHOL BLUE IN LIQUID WASTE MANAGEMENT IN THE SONGKET, BLONGKET, AND JUMPUTAN WEAVING INDUSTRY IN PALEMBANG

NURHAYATI 1, HILDA ZULKIFLI 2, and RISFIDIAN MOHADI 3.

Vol 18, No 05 ( 2023 )   |  DOI: 10.17605/OSF.IO/TWZGR   |   Author Affiliation: Master Student of Biology Sriwijaya University, JL. Raya Palembang-Prabumulih KM.32 Indralaya, OI, South Sumatra 1; Supervisors of Master of Biology Sriwijaya University, JL. Raya Palembang-Prabumulih KM.32 Indralaya, OI, South Sumatra 2; Supervisors of Master of Chemistry, Sriwijaya University, JL. Raya Palembang-Prabumulih KM.32 Indralaya, OI, South Sumatra 3.   |   Licensing: CC 4.0   |   Pg no: 2056-2073   |   Published on: 31-05-2023

Abstract

This study aims to determine the growth of Spirulina sp. in culture media with the addition of heavy metals Fe and Zn with degradation cycles of Procion Red, Methyl Orange, Direct Green, and Napthol Blue. The method used is experimental by taking Spirulina sp. microalgae. Obtained from ponds in Palembang and Prabumulih, South Sumatra with laboratory-scale algae culture. The design in this study used a completely randomized design (CRD). The treatment used for each metal Fe, Zn and dyes Procion Red, Methyl Orange, Direct Green and Napthol Blue was 5 treatments with 3 replications, in 1 L medium containing Spirulina sp. with an individual average of 1×104 ind/L. Method validation for heavy metals Fe and Zn was calculated with AAS (Atomic Absor Btion Spectrophotometer) and dyes with Lambert Beer isotherm, SEM and FTIR tests. The results showed that the growth of Spirulina sp. in the control is greater than in the metal treatment with a span of 14 days. The average growth size of Spirulina in the Fe control was 652.62 ind/L, Zn was 636.3 ind/L and the decrease in Fe metal at 1, 3, 5, 7 ppm was 33%, 10%, 5.6%, 4 respectively. .28%. While the decrease in Zn metal at 1, 3, 5, 7 ppm was 15%, 8.67%, 4.2%, 4%. Furthermore, the growth of Spirulina sp. in the control dyes Procion red, Methyl Orange, Direct Green, Napthol Blue, namely 643.44 ind/L, 631.08 ind/L, 634.34 ind/L, 638.08 ind/L. Then the decrease in dyes in Procion Red at concentrations of 1, 3, 5, 7 ppm, namely 90.7%, 76.33%, 72%, 64.14%. Furthermore, the decrease in Methyl Orange 1, 3, 5, 7 ppm is 71.1%, 69.67%, 79.2%, 88.14%. In addition, the reduction of Direct Green dye at 1, 3, 5, 7 ppm was 36%, 28.33%, 48%, 81.42%. Furthermore, the decrease in Napthol Blue dye at 1, 3, 5, 7 ppm was 12.6%, 49%, 55.4%, 47.85%. FTIR test results of Spirulina sp. shows three sharp peaks which are in the absorption area 3259.65 cm-1 which is the OH group (Hydroxyl), Absorption which appears in the area 1640.76 cm-1 which is the aromatic C=C group, at wave number 1020.74 is the C-O group, 547.32 is a cluster of fingerprint regions.


Keywords

Spirulina sp., Heavy Metals Fe, Zn, Water pollution, Textile dyes Procion Red, Methyl Orange, Direct Green, Napthol Blue