ASSESSMENT OF MUTAGENIC EFFECTIVENESS AND EFFICIENCY OF EMS AND GAMMA RAYS IN VICIA FABA L. VAR. PRT-12
The success of any mutation breeding programme for genetic improvement of crop plant depends on the effectiveness and efficiency of the mutagen used. In the present investigation five different concentrations of ethyl methane sulphonate (EMS) i.e., 0.05, 0.75, 0.10 and 0.25 and 0.50mol/mL and 50Gy, 100Gy, 150Gy, 200Gy and 250Gy of gamma rays were used to induce genetic variations Vicia faba L. var. PRT-12 which can be used as viable and economically important character in future breeding programme of this crop. The standard formulae were used to quantify the mutagenic efficiency and effectiveness of the EMS and gamma rays. Overall, the findings showed that as mutagenic treatments increased, so did their efficacy and efficiency. Furthermore, it was discovered that EMS and lower-or intermediate-level gamma-ray treatments were more successful in producing the greatest number of mutations while causing the least amount of biological damage.
EMS, Gamma Rays, Mutagenesis, Genetic Improvement, Vicia faba.