INVESTIGATION OF PROTEIN PATTERNS BY USING SDS-PAGE IN INTESTINE TISSUE EXPOSED TO MALATHION (OP) OF TWO FRESH WATER FISHES CHANNA PUNCTATUS AND LABEO ROHITA
Pesticides find a perennial application in agriculture, combating pest and enhancing productivity. But on the rear end, these pesticides find avenues to enter water bodies thereby affecting aquatic flora and fauna. These pesticides cause significant variation in protein particularly in fish. Present study focused on the effect of Malathion (LC50) induced protein profiling and alteration in the intestine tissue of freshwater fishes Channa punctatus and Labeo rohita at different time periods i.e. 24H, 48H, 72H and 96H. And results shown that 7 protein bands in Channa punctatus and 9 protein bands in Labeo rohita controls the functions of intestine tissue of both the fishes, and also observed that prominent decrease in protein in the test period. SDS-PAGE analysis revealed decreased in intensity and the formation/deletion of bands of the treated tissues with Malathion when compared to control was due to pesticidal stress. More research should be performed to conclude the lethal and sub-lethal impact of the particular pesticide used on the survival and performance of non-target invertebrate and vertebrate animals including fish health.
Channa punctatus, Labeo rohita Pesticide, Malathion, Protein Profile, SDS-PAGE, Intestine Tissue